Affinity Chromatography and Binding Studies on Immobilized Adenosine 5'-Monophosphate and Adenosine 2',5'-Bisphosphateof Nicotinamide Nucleotide Transhydrogenasefrom Pseudomonas aeruginosa
نویسندگان
چکیده
منابع مشابه
The Determination of Dissociation Constants by Affinity Chromatography on an Immobilized Adenosine Monophosphate Analogue
Within a few years affinity chromatography has become a widely used technique for the separation and purification of enzymes. The development and advantages of general ligands in affinity chromatography have previously been reported (Mosbach et al., 1972; Ohlsson et al., 1972). Thus Sepharose-bound N6-(6-aminohexyl)-AMP has been used for the separation and purification of NAD+-dependent enzymes...
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A new adenine nucleotide analog, [3H]pyridoxal 5'-diphospho-5'-adenosine (PLP-AMP), has been synthesized. The effectiveness of PLP-AMP as an affinity probe has been tested using a number of nucleotide-binding enzymes. In comparison to reaction with pyridoxal 5'-phosphate, PLP-AMP binds more tightly and exhibits greater specificity of labeling for most enzymes tested. PLP-AMP is a very potent in...
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The adenine nucleotides, 5'-AMP and 3',5'-cyclic AMP block L cells in the S-phase of the cell cycle. The intracellular level of cyclic AMP is reduced after incubation of cells with 5'-AMP, and rates of uridine transport are increased after incubation with either 5'-AMP or cyclic AMP. On the contrary, cyclic AMP levels are increased and uridine transport decreased in cells treated with an inhibi...
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Three derivatives of cyclic AMP that are potentially useful as photo-affinity labels were synthesized. One of these derivatives is, upon photolysis, specifically incorporated into the cyclic AMP-binding site of rabbit muscle phosphofructokinase. The use of these derivatives in isolating and identifying cyclic AMP receptor sites is discussed.
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An abstract of this work appeared in 1970 Abstracts, The American Society of Nephrology. Received for publication 27 October 1970 and in revised form 28 January 1971. similar to the effect of renal arterial infusion of isoproterenol. The results suggest that synthesis of cyclic AMP in proximal renal tubule cells in response to stimulation of beta adrenergic or other receptors can mediate a decr...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1976
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1976.tb10571.x